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hexanucleotide random primers
Hexanucleotide Random Primers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hexanucleotide+random+primers/Random+Primer/pmc11061733-52-24-27
Average 96 stars, based on 6512 article reviews
Hexanucleotide Random Primers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hexanucleotide+random+primers/Random+Primer/pmc11061733-52-24-27
Average 96 stars, based on 6512 article reviews
hexanucleotide random primers - by Bioz Stars,
2026-09
96/100 stars
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Reverse Transcription:Article Title: The effects of sevoflurane anesthesia on rat hippocampus: a genomic expression analysis. Article Snippet: Article history: Accepted 8 January 2011 Available online 15 January 2011 Recentstudieshaveshownthatgeneral anesthesia inducesmemory impairment.. Sevoflurane, an inhalation anesthetic, is widely used in clinical practice, increasing pieces of evidence suggest that sevoflurane impairs memory processes due to changing gene expression in hippocampus.However, little isknownabout genome-widely analyzing the expressionchange induced by sevoflurane inhippocampus.. In this study,weprofiled the changes ofhippocampal gene expression by microarray analysis. Article Title: A prostaglandin E2 receptor subtype EP4 agonist attenuates cardiovascular depression in endotoxin shock by inhibiting inflammatory cytokines and nitric oxide production. Article Snippet: The prostaglandin (PG) E2 receptor subtype EP4 has been found to mediate regulation of inflammatory cytokines in macrophages and neutrophils in vitro by PGE2.. Yet the role of EP4 receptors in endotoxin shock in vivo and whether EP4 activation is a beneficial treatment are not clear.. We tested the effect of an EP4 agonist on hemodynamic changes and production of inflammatory cytokines in a rat endotoxin-induced shock model. Article Title: Circadian gene expression is suppressed during sevoflurane anesthesia and the suppression persists after awakening. Article Snippet: Article history: Accepted 9 September 2007 Available online 16 September 2007 General anesthesia is routinely used as a surgical procedure and its safety has been endorsed by clinical outcomes; however, its effects at themolecular level have not been elucidated.We previously showed that inhalation anesthesia affects the expression of 1.5% of 10,000 genes, which included so-called circadian genes.. In the current study, we confirmed that inhalation of sevoflurane alters circadian gene expression, and investigated whether this alteration persists after awakening from anesthesia.. Rats were anesthetized with 4.0% sevoflurane for 0 h, 2 h and 6 h (n=9 each group), before being sacrificed. Article Title: Pivotal roles of interleukin-6 in transmural inflammation in murine T cell transfer colitis. Article Snippet: RNA isolation and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) Total RNA was extracted from a part of colon tissues with RNA-BeeTM (Tel-Test Inc., Friendswood, TX), according to the manufacturer’s instructions, and cDNA was synthesized as described previously [23]. .. Briefly, 2 g total RNA was reverse-transcribed at 30°C for 10 min, at 42°C for 60 min, and at 99°C for 5 min in 20 l reaction mixture containing 100 units ReverTra Ace (Toyobo, Osaka, Japan) and 50 pmol Article Title: Changes in expression of GABAA alpha4 subunit mRNA in the brain under anesthesia induced by volatile and intravenous anesthetics. Article Snippet: We investigated changes in levels of GABAA receptor a4 subunit mRNA in the mouse brain after administration of volatile or i.v. anesthetic, by performing quantitative RT-PCR.. We also performed immunohistochemical assays for c-fos-like protein.. During deep anesthesia (which was estimated by loss of righting reflex) after administration of propofol, levels of GABAA receptor a4 subunit mRNA in the hippocampus, striatum and diencephalons were significantly greater than those observed after administration of pentobarbital, midazolam or GOI (5.0% isoflurane and 70% nitrous oxide in oxygen). Article Title: Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKα-regulated autophagy induction in streptozotocin-induced diabetic nephropathy Article Snippet: Total RNA was extracted from renal cortex using Trizol reagent (Invitrogen, Carlsbad, CA, USA). .. Then first strand cDNAs were synthesized from 1 μg of total RNA in a 20 μl reaction volume using Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Ipswich, MA, USA) and Article Title: Increases in COX II mRNA in the rat spinal cord induced by cauda equina traction. Article Snippet: Intern.. J. Neuroscience, 116:575–586, 2006 Copyright C© 2006 Taylor & Francis Group, LLC ISSN: 0020-7454 / 1543-5245 online DOI: 10.1080/00207450600592115 INCREASES IN COX II mRNA IN THE RAT SPINAL CORD INDUCED BY CAUDA EQUINA TRACTION KIYOKO HIRABAYASHI Department of Anesthesiology Tokyo Medical University Shinjuku, Tokyo, Japan MASASHI KOMAGATA Department of Orthopedics Tokyo Medical University Shinjuku, Tokyo, Japan JINZO YAMADA Department of Anatomy Tokyo Medical University Shinjuku, Tokyo, Japan ATSUSHI ISSHIKI Department of Anesthesiology Tokyo Medical University Shinjuku, Tokyo, Japan YASUO WATANABE Department of Pharmacology Tokyo Medical University Shinjuku, Tokyo, Japan Received 18 February 2005.. Address correspondence to Dr. Yasuo Watanabe, Department of Pharmacology and Pharmacotherapy, School of Pharmaceutical Sciences, Nihon Pharmaceutical University, 10281, Komuro, Ina-machi, Saitama 362-0806, Japan. Synthesized:Article Title: Prevalence and species diversity of dsRNA mycoviruses from Beauveria bassiana strains in the China's Guniujiang nature Article Snippet: To generate viral fragments, contigs corresponding to the same viruses were assembled using DNAMAN 7.0 (Lynnon Biosoft, USA) software. .. To confirm the presence of putative mycoviruses in the strains, cDNA was synthesized using Moloney murine leukemia virus transcriptase (M-MLV, Takara Dalian, China) and Article Title: Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKα-regulated autophagy induction in streptozotocin-induced diabetic nephropathy Article Snippet: Total RNA was extracted from renal cortex using Trizol reagent (Invitrogen, Carlsbad, CA, USA). .. Then first strand cDNAs were synthesized from 1 μg of total RNA in a 20 μl reaction volume using Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Ipswich, MA, USA) and Virus:Article Title: Prevalence and species diversity of dsRNA mycoviruses from Beauveria bassiana strains in the China's Guniujiang nature Article Snippet: To generate viral fragments, contigs corresponding to the same viruses were assembled using DNAMAN 7.0 (Lynnon Biosoft, USA) software. .. To confirm the presence of putative mycoviruses in the strains, cDNA was synthesized using Moloney murine leukemia virus transcriptase (M-MLV, Takara Dalian, China) and Article Title: A prostaglandin E2 receptor subtype EP4 agonist attenuates cardiovascular depression in endotoxin shock by inhibiting inflammatory cytokines and nitric oxide production. Article Snippet: The prostaglandin (PG) E2 receptor subtype EP4 has been found to mediate regulation of inflammatory cytokines in macrophages and neutrophils in vitro by PGE2.. Yet the role of EP4 receptors in endotoxin shock in vivo and whether EP4 activation is a beneficial treatment are not clear.. We tested the effect of an EP4 agonist on hemodynamic changes and production of inflammatory cytokines in a rat endotoxin-induced shock model. Article Title: Circadian gene expression is suppressed during sevoflurane anesthesia and the suppression persists after awakening. Article Snippet: Article history: Accepted 9 September 2007 Available online 16 September 2007 General anesthesia is routinely used as a surgical procedure and its safety has been endorsed by clinical outcomes; however, its effects at themolecular level have not been elucidated.We previously showed that inhalation anesthesia affects the expression of 1.5% of 10,000 genes, which included so-called circadian genes.. In the current study, we confirmed that inhalation of sevoflurane alters circadian gene expression, and investigated whether this alteration persists after awakening from anesthesia.. Rats were anesthetized with 4.0% sevoflurane for 0 h, 2 h and 6 h (n=9 each group), before being sacrificed. Article Title: Changes in expression of GABAA alpha4 subunit mRNA in the brain under anesthesia induced by volatile and intravenous anesthetics. Article Snippet: We investigated changes in levels of GABAA receptor a4 subunit mRNA in the mouse brain after administration of volatile or i.v. anesthetic, by performing quantitative RT-PCR.. We also performed immunohistochemical assays for c-fos-like protein.. During deep anesthesia (which was estimated by loss of righting reflex) after administration of propofol, levels of GABAA receptor a4 subunit mRNA in the hippocampus, striatum and diencephalons were significantly greater than those observed after administration of pentobarbital, midazolam or GOI (5.0% isoflurane and 70% nitrous oxide in oxygen). Article Title: Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKα-regulated autophagy induction in streptozotocin-induced diabetic nephropathy Article Snippet: Total RNA was extracted from renal cortex using Trizol reagent (Invitrogen, Carlsbad, CA, USA). .. Then first strand cDNAs were synthesized from 1 μg of total RNA in a 20 μl reaction volume using Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Ipswich, MA, USA) and Article Title: Increases in COX II mRNA in the rat spinal cord induced by cauda equina traction. Article Snippet: Intern.. J. Neuroscience, 116:575–586, 2006 Copyright C© 2006 Taylor & Francis Group, LLC ISSN: 0020-7454 / 1543-5245 online DOI: 10.1080/00207450600592115 INCREASES IN COX II mRNA IN THE RAT SPINAL CORD INDUCED BY CAUDA EQUINA TRACTION KIYOKO HIRABAYASHI Department of Anesthesiology Tokyo Medical University Shinjuku, Tokyo, Japan MASASHI KOMAGATA Department of Orthopedics Tokyo Medical University Shinjuku, Tokyo, Japan JINZO YAMADA Department of Anatomy Tokyo Medical University Shinjuku, Tokyo, Japan ATSUSHI ISSHIKI Department of Anesthesiology Tokyo Medical University Shinjuku, Tokyo, Japan YASUO WATANABE Department of Pharmacology Tokyo Medical University Shinjuku, Tokyo, Japan Received 18 February 2005.. Address correspondence to Dr. Yasuo Watanabe, Department of Pharmacology and Pharmacotherapy, School of Pharmaceutical Sciences, Nihon Pharmaceutical University, 10281, Komuro, Ina-machi, Saitama 362-0806, Japan. Quantitative RT-PCR:Article Title: Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKα-regulated autophagy induction in streptozotocin-induced diabetic nephropathy Article Snippet: Total RNA was extracted from renal cortex using Trizol reagent (Invitrogen, Carlsbad, CA, USA). .. Then first strand cDNAs were synthesized from 1 μg of total RNA in a 20 μl reaction volume using Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Ipswich, MA, USA) and Real-time Polymerase Chain Reaction:Article Title: Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKα-regulated autophagy induction in streptozotocin-induced diabetic nephropathy Article Snippet: Total RNA was extracted from renal cortex using Trizol reagent (Invitrogen, Carlsbad, CA, USA). .. Then first strand cDNAs were synthesized from 1 μg of total RNA in a 20 μl reaction volume using Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Ipswich, MA, USA) and SYBR Green Assay:Article Title: Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKα-regulated autophagy induction in streptozotocin-induced diabetic nephropathy Article Snippet: Total RNA was extracted from renal cortex using Trizol reagent (Invitrogen, Carlsbad, CA, USA). .. Then first strand cDNAs were synthesized from 1 μg of total RNA in a 20 μl reaction volume using Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Ipswich, MA, USA) and Sequencing:Article Title: Increases in COX II mRNA in the rat spinal cord induced by cauda equina traction. Article Snippet: Intern.. J. Neuroscience, 116:575–586, 2006 Copyright C© 2006 Taylor & Francis Group, LLC ISSN: 0020-7454 / 1543-5245 online DOI: 10.1080/00207450600592115 INCREASES IN COX II mRNA IN THE RAT SPINAL CORD INDUCED BY CAUDA EQUINA TRACTION KIYOKO HIRABAYASHI Department of Anesthesiology Tokyo Medical University Shinjuku, Tokyo, Japan MASASHI KOMAGATA Department of Orthopedics Tokyo Medical University Shinjuku, Tokyo, Japan JINZO YAMADA Department of Anatomy Tokyo Medical University Shinjuku, Tokyo, Japan ATSUSHI ISSHIKI Department of Anesthesiology Tokyo Medical University Shinjuku, Tokyo, Japan YASUO WATANABE Department of Pharmacology Tokyo Medical University Shinjuku, Tokyo, Japan Received 18 February 2005.. Address correspondence to Dr. Yasuo Watanabe, Department of Pharmacology and Pharmacotherapy, School of Pharmaceutical Sciences, Nihon Pharmaceutical University, 10281, Komuro, Ina-machi, Saitama 362-0806, Japan. |